Eukaryotic N-glycosylation Occurs Via Membrane-Anchored C-terminal domain of Stt3p subunit of Oligosaccharyl Transferase*
نویسندگان
چکیده
Background: Stt3p is the catalytic subunit of Oligosaccharyl Transferase (OT) that catalyses protein N-glycosylation. Results: We report the first high-resolution NMR structure of the acceptor binding domain of yeast OT. Conclusion: This work provides structural basis for the function of the C-terminal Domain of Stt3p subunit. Significance: Structure determination of this critical domain is an important step towards understanding the mechanisms of the eukaryotic N-glycosylation process.
منابع مشابه
Studies on the N-glycosylation of the subunits of oligosaccharyl transferase in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, oligosaccharyl transferase (OT) consists of nine different subunits. Three of the essential gene products, Ost1p, Wbp1p, and Stt3p, are N-linked glycoproteins. To study the function of the N-glycosylation of these proteins, we prepared single or multiple N-glycosylation site mutations in each of them. We established that the four potential N-glycosylation sites in O...
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تاریخ انتشار 2012